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Overexpressing lysates

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Western blot validation of overexpression lysate (Cat# LY400178) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC202450 using transfection reagent MegaTran 2.0 (Cat# TT210002).
Product group Lysates / Extracts
OriGene
Myelin Protein Zero (MPZ) (NM_000530) Human Over-expression LysateLC400178
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Western blot validation of overexpression lysate (Cat# LY426997) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC225379 using transfection reagent MegaTran 2.0 (Cat# TT210002).
Product group Lysates / Extracts
OriGene
PLP1 (NM_000533) Human Over-expression LysateLC400181
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Western blot validation of overexpression lysate (Cat# LY400182) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC208589 using transfection reagent MegaTran 2.0 (Cat# TT210002).
Product group Lysates / Extracts
OriGene
Prothrombin (F2) (NM_000506) Human Over-expression LysateLC400182
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Western blot validation of overexpression lysate (Cat# LY400183) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC200006 using transfection reagent MegaTran 2.0 (Cat# TT210002).
Product group Lysates / Extracts
OriGene
LDL Receptor (LDLR) (NM_000527) Human Over-expression LysateLC400183
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Western blot validation of overexpression lysate (Cat# LY400184) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC211328 using transfection reagent MegaTran 2.0 (Cat# TT210002).
Product group Lysates / Extracts
OriGene
Rhodopsin (RHO) (NM_000539) Human Over-expression LysateLC400184
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Western blot validation of overexpression lysate (Cat# LY400185) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC211201 using transfection reagent MegaTran 2.0 (Cat# TT210002).
Product group Lysates / Extracts
OriGene
HNF1 alpha (HNF1A) (NM_000545) Human Over-expression LysateLC400185
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TP53 activity in the over-expression lysate (LY400186) was measured in a colorimetric DNA-binding assay. Double-stranded oligonucleotide containing the p53 consensus DNA-binding sequence was incubated with dilutions of the over-expression lysate and TP53 bound to the oligo was captured onto the surface of a microtiter plate. After washing, bound TP53 was detected with an anti-p53 primary antibody followed by an HRP-labeled secondary antibody. After initial color development, the reaction was quenched and the color intensity was measured at 450nm. Overexpression cell lysates are prepared from HEK293T cells transfected with RC200003 using transfection reagent MegaTran 2.0 (Cat# TT210002).
Product group Lysates / Extracts
OriGene
p53 (TP53) (NM_000546) Human Over-expression LysateLC400186
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Western blot validation of overexpression lysate (Cat# LY400187) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC208595 using transfection reagent MegaTran 2.0 (Cat# TT210002).
Product group Lysates / Extracts
OriGene
TRP1 (TYRP1) (NM_000550) Human Over-expression LysateLC400187
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Western blot validation of overexpression lysate (Cat# LY400188) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC216151 using transfection reagent MegaTran 2.0 (Cat# TT210002).
Product group Lysates / Extracts
OriGene
Von Hippel Lindau (VHL) (NM_000551) Human Over-expression LysateLC400188
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Western blot validation of overexpression lysate (Cat# LY400189) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC203259 using transfection reagent MegaTran 2.0 (Cat# TT210002).
Product group Lysates / Extracts
OriGene
HBA1 (NM_000558) Human Over-expression LysateLC400189
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Western blot validation of overexpression lysate (Cat# LY400190) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC203084 using transfection reagent MegaTran 2.0 (Cat# TT210002).
Product group Lysates / Extracts
OriGene
hemoglobin subunit gamma 1 (HBG1) (NM_000559) Human Over-expression LysateLC400190
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Western blot validation of overexpression lysate (Cat# LY400191) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC219758 using transfection reagent MegaTran 2.0 (Cat# TT210002).
Product group Lysates / Extracts
OriGene
Acid sphingomyelinase (SMPD1) (NM_000543) Human Over-expression LysateLC400191
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