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Western Blot Positive WB detected in: Mouse heart tissue, Mouse kidney tissue, Mouse brain tissue All lanes: OVGP1 antibody at 3ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 76 kDa Observed band size: 76 kDa
Western Blot Positive WB detected in: Mouse heart tissue, Mouse kidney tissue, Mouse brain tissue All lanes: OVGP1 antibody at 3ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 76 kDa Observed band size: 76 kDa
Western Blot Positive WB detected in: Mouse heart tissue, Mouse kidney tissue, Mouse brain tissue All lanes: OVGP1 antibody at 3ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 76 kDa Observed band size: 76 kDa

OVGP1 Antibody

CSB-PA614260LA01HU
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse
TargetOVGP1
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Overview

  • Supplier
    Cusabio
  • Product Name
    OVGP1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5016
  • Target name
    OVGP1
  • Target description
    oviductal glycoprotein 1
  • Target synonyms
    CHIT5, EGP, MUC9, OGP, oviduct-specific glycoprotein, estrogen-dependent oviduct protein, mucin 9, oviduct glycoprotein, oviductal glycoprotein 1, 120kDa, oviductin
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ12889
  • Protein Name
    Oviduct-specific glycoprotein
  • Scientific Description
    Binds to oocyte zona pellucida in vivo. May play a role in the fertilization process and/or early embryonic development.
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of OVGP1 using anti-OVGP1 antibody (A07911-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human RT4 whole cell lysates, Lane 4: human PC-3 whole cell lysates, Lane 5: rat testis tissue lysates, Lane 6: mouse ovary tissue lysates, Lane 7: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-OVGP1 antigen affinity purified polyclonal antibody (Catalog # A07911-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for OVGP1 at approximately 75 kDa. The expected band size for OVGP1 is at 75 kDa.
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