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ProductsBack/OXCT2 antibody, Internal
WB analysis of human fetal lung tissue using GTX46174 OXCT2 antibody at 1microg/ml.
WB analysis of human fetal lung tissue using GTX46174 OXCT2 antibody at 1microg/ml.
WB analysis of human fetal lung tissue using GTX46174 OXCT2 antibody at 1microg/ml.

OXCT2 antibody, Internal

GTX46174
GeneTex
ApplicationsWestern Blot
Product group Antibodies
TargetOXCT2
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Overview

  • Supplier
    GeneTex
  • Product Name
    OXCT2 antibody, Internal
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 0.2-2.5 ug/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5-1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID64064
  • Target name
    OXCT2
  • Target description
    3-oxoacid CoA-transferase 2
  • Target synonyms
    3-oxoacid CoA-transferase 2A; FKSG25; SCOTT; succinyl-CoA:3-ketoacid coenzyme A transferase 2, mitochondrial; testis-specific succinyl CoA:3-oxoacid CoA-transferase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9BYC2
  • Protein Name
    Succinyl-CoA:3-ketoacid coenzyme A transferase 2, mitochondrial
  • Scientific Description
    The protein encoded by this gene catalyzes the transfer of a CoA group from succinate to acetoacetate and is an important enzyme in ketone body catabolism. The encoded protein localizes to the mitochondrion. This gene is intronless, and a pseudogene of this gene is located elsewhere on chromosome 1. [provided by RefSeq, Aug 2016]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of OXCT2 using anti-OXCT2 antibody (A15113-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-OXCT2 antigen affinity purified polyclonal antibody (Catalog # A15113-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for OXCT2 at approximately 56 kDa. The expected band size for OXCT2 is at 56 kDa.
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