OxiSelect Intracellular ROS Assay Kit (Green Fluorescence)

Cell Biolabs

OxiSelect Intracellular ROS Assay Kit (Green Fluorescence)

4

Fluorometric

Kit

Research Use Only

The OxiSelect ROS Assay Kit is a cell-based assay for measuring hydroxyl, peroxyl, and other reactive oxygen species activity within a cell. The assay employs the cell-permeable fluorogenic probe DCFH-DA, which diffuses into cells and is deacetylcated by cellular esterases into the non-fluorescent DCFH (Figure 1). In the presence of ROS, DCFH is rapidly oxidized to highly fluorescent DCF. Fluorescence is read on a standard fluorometric plate reader.

Upon receipt, store the DCFH-DA, DCF and Hydrogen Peroxide at -20ºC. Avoid multiple freeze/thaw cycles. Store the Cell Lysis Buffer at 4ºC.

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