OxiSelect Nepsilon-(carboxyethyl) lysine (CEL) Competitive ELISA Kit

Cell Biolabs

OxiSelect Nepsilon-(carboxyethyl) lysine (CEL) Competitive ELISA Kit

4

ELISA

Colorimetric

Kit

Research Use Only

First, a CEL conjugate is coated on the ELISA plate.  The unknown CEL protein samples or CEL-BSA standards are then added to the CEL conjugate preabsorbed plate.  After a brief incubation, the anti-CEL monoclonal antibody is added, followed by an HRP conjugated secondary antibody.  The content of CEL protein adducts in unknown samples is determined by comparison with the predetermined CEL-BSA standard curve.  Despite the structure similarity between CEL and CML, the anti-CEL specific antibody in the OxiSelect CEL ELISA Kit will not cross react with CML protein adducts.

Upon receipt, aliquot and store the CEL-BSA Standard, CEL Conjugate and 100X Conjugate Diluent at -20ºC to avoid multiple freeze/thaw cycles. Store all other kit components at 4ºC.

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