OxiSelect Nepsilon-(carboxymethyl) lysine (CML) Competitive ELISA Kit

Cell Biolabs

OxiSelect Nepsilon-(carboxymethyl) lysine (CML) Competitive ELISA Kit

4

ELISA

Colorimetric

Kit

Research Use Only

Advanced glycation end products (AGE) are formed during the Maillard reaction where reducing carbohydrates react with lysine side chains and N-terminal amino groups of various macromolecules, particularly proteins. The advanced glycation end products can adversely affect the fuction of these macromolecules. One of the most prevalent AGE products, N-epsilon-(Carboxymethyl) Lysine, has been implicated in oxidative stress and vascular damage. The OxiSelect N-epsilon-(Carboxymethyl) Lysine Competitive ELISA kit specifically detects CML formation with a high level of sensitivity. This is a Competitive ELISA Kit in which the plate is coated with a CML conjugate.  Standards and unknown samples are added to the plate, followed by incubation with the primary antibody. The CML in the unknown samples and the CML attached to the plate compete for the primary antibody. Higher CML content in unknown samples results in more binding of the antibody to the sample, and thus less antibody binds to the plate. Since the antibody bound to the sample is washed away, higher CML content in samples correlates with a lower signal.

Upon receipt, aliquot and store the CML-BSA Standard, CML Conjugate and 100X Conjugate Diluent at -20ºC to avoid multiple freeze/thaw cycles. Store all other kit components at 4ºC.

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