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OXSM / KS Antibody

LS-C816897
LifeSpan BioSciences
ApplicationsImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetOXSM
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    OXSM / KS Antibody
  • Delivery Days Customer
    14
  • Application Supplier Note
    Applications should be user optimized.
  • Applications
    ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    IHC-P (1:50 - 1:200) Applications should be user optimized.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID54995
  • Target name
    OXSM
  • Target description
    3-oxoacyl-ACP synthase, mitochondrial
  • Target synonyms
    CEM1, FASN2D, KASI, KS, 3-oxoacyl-[acyl-carrier-protein] synthase, mitochondrial, 3-ketoacyl-acyl carrier protein synthase, 3-oxoacyl-acyl-carrier-protein synthase, acyl-malonyl acyl carrier protein-condensing enzyme, beta-ketoacyl synthase, beta-ketoacyl-ACP synthase, type II mitochondrial beta-ketoacyl synthase
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

FAQ

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Figure 1. Western blot analysis of OXSM using anti-OXSM antibody (A12866-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: rat heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-OXSM antigen affinity purified polyclonal antibody (Catalog # A12866-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for OXSM at approximately 46 kDa. The expected band size for OXSM is at 46,49 kDa.
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