oYo-Link Azide-TAMRA Antibody Labeling Reagent - Labels 100ug

AlphaThera

oYo-Link Azide-TAMRA Antibody Labeling Reagent - Labels 100ug

9

Cytotoxic Assay

Click Chemistry reactions with DBCO-labeled molecules or surface immobilization (e.g. DBCO functional nanoparticle) before antibody conjugation, then fluorescent imaging and/or quantification of antibodies

Research Use Only

270 ug/mL

TAMRA

Dried pellet

NOTE: It is important to perform the click-reaction with DBCO-x first, prior to antibody conjugation. UV illumination will damage the Azide activity and thus antibody conjugation should only be performed after the click-reaction. Research Use Only Applications: Click Chemistry; Fluorescent imaging and/or quantification of oYo-Link following conjugation or surface immobilization (e.g. to nanoparticles) oYo-Link Azide allows for the site-specific labeling of oYo-Link-compatible antibodies with up to two azide labels per antibody for subsequent click chemistry reactions. oYo-Link Azide limits the attachment of the azide labels to the heavy chains of the antibody and therefore ensures that the azide label does not interfere with antibody binding. Furthermore, site-specific labeling limits 1-2 azide labels to be attached per antibody, providing precise antibody-conjugate ratios.The antibody labeling procedure simply requires mixing oYo-Link Azide with the desired antibody followed by photocrosslinking with non-damaging black light (365 nm). Photocrosslinking can be carried out in most buffers, including those containing amine-containing molecules (e.g. TRIS, glycine) or storage proteins (see Buffer Compatibility Table) . Requirements: To label your compatible antibodies with oYo-Link, you will need a Light Source emitting at 365nm (6-10W). Use your own Compatible Device from our list or purchase AlphaThera's LED Photo-Crosslinking Device. Technical Support: For questions concerning compatibility or click-chemistry procedure email support@alphathera.com

4°C for short-term durations (< 1 week) and -20°C to -80°C for longer time-spans

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