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oYo-Link® Azide Product Icon
oYo-Link® Azide Product Icon
oYo-Link® Azide Product Icon

oYo-Link mIgG1 Azide Antibody Labeling Reagent - Labels 1mg mIgG1 Ab

Research Use Only
at3002-migg1
AlphaThera
Product group Antibodies
Price on request
Packing Size
Large volume orders?
Order with a bulk request

Overview

  • Supplier
    AlphaThera
  • Product Name
    oYo-Link mIgG1 Azide Antibody Labeling Reagent - Labels 1mg mIgG1 Ab
  • Delivery Days Customer
    9
  • Applications Supplier
    Click Chemistry reactions with DBCO-labeled molecules
  • Certification
    Research Use Only
  • Concentration
    270 ug/mL
  • Format
    Dried pellet
  • Scientific Description
    NOTE: It is important to perform the click-reaction with DBCO-x first, prior to antibody conjugation. UV illumination will damage the Azide activity and thus antibody conjugation should only be performed after the click-reaction. Research Use Only Applications: Click Chemistry oYo-Link Azide allows for the site-specific labeling of oYo-Link-compatible antibodies with up to two azide labels per antibody for subsequent click chemistry reactions. oYo-Link Azide limits the attachment of the azide labels to the heavy chains of the antibody and therefore ensures that the azide label does not interfere with antibody binding. Furthermore, site-specific labeling limits 1-2 azide labels to be attached per antibody, providing precise antibody-conjugate ratios.The antibody labeling procedure simply requires mixing oYo-Link Azide with the desired antibody followed by photocrosslinking with non-damaging black light (365 nm). Photocrosslinking can be carried out in most buffers, including those containing amine-containing molecules (e.g. TRIS, glycine) or storage proteins (see Buffer Compatibility Table) . Requirements: To label your compatible antibodies with oYo-Link, you will need a Light Source emitting at 365nm (6-10W). Use your own Compatible Device from our list or purchase AlphaThera's LED Photo-Crosslinking Device. Technical Support: For questions concerning compatibility or click-chemistry procedure email support@alphathera.com
  • Storage Instruction
    4°C for short-term durations (< 1 week) and -20°C to -80°C for longer time-spans
  • UNSPSC
    12352203