Whole cell extract (30 microg) was separated by 5% SDS-PAGE, and the membrane was blotted with P Glycoprotein antibody [C219] (GTX23364) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Whole cell extract (30 microg) was separated by 5% SDS-PAGE, and the membrane was blotted with P Glycoprotein antibody [C219] (GTX23364) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
P Glycoprotein antibody [C219]
GTX23364
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityBovine, Canine, Fish, Human, Mouse, Rat, Zebra Fish
TargetABCB1
Overview
- SupplierGeneTex
- Product NameP Glycoprotein antibody [C219]
- Delivery Days Customer9
- Antibody SpecificityRecognizes an internal, highly conserved amino acid sequence (VQEALD and VQAALD) found in both protein isoforms, P-glycoprotein and MDR3 P-glycoprotein. Specific reactivity to human, mouse, rat, dog and primate. Detects bands of ~170kDa (MDR1 P-glycoprote
- Application Supplier NoteWB: 1:50-1:500. IHC-P: ≥1:25. FACS: . *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDC219
- ConjugateUnconjugated
- Gene ID5243
- Target nameABCB1
- Target descriptionATP binding cassette subfamily B member 1
- Target synonymsABC20; ATP-binding cassette, sub-family B (MDR/TAP), member 1; ATP-dependent translocase ABCB1; CD243; CLCS; colchicin sensitivity; doxorubicin resistance; GP170; MDR1; multidrug resistance protein 1; p-170; P-glycoprotein 1; P-GP; PGY1; phospholipid transporter ABCB1
- HostMouse
- IsotypeIgG1
- Protein IDP08183
- Protein NameMultidrug resistance protein 1
- Scientific DescriptionThe membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance. The protein encoded by this gene is an ATP-dependent drug efflux pump for xenobiotic compounds with broad substrate specificity. It is responsible for decreased drug accumulation in multidrug-resistant cells and often mediates the development of resistance to anticancer drugs. This protein also functions as a transporter in the blood-brain barrier. Mutations in this gene are associated with colchicine resistance and Inflammatory bowel disease 13. Alternative splicing and the use of alternative promoters results in multiple transcript variants. [provided by RefSeq, Feb 2017]
- ReactivityBovine, Canine, Fish, Human, Mouse, Rat, Zebra Fish
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Loss of Blood-Brain Barrier Integrity in an In Vitro Model Subjected to Intermittent Hypoxia: Is Reversion Possible with a HIF-1alpha Pathway Inhibitor?Read more
- Blood-brain barrier-restricted translocation of Toxoplasma gondii from cortical capillaries. Olivera GC et al., 2021 Dec 8, ElifeRead more
- USP24 promotes drug resistance during cancer therapy. Wang SA et al., 2021 Sep, Cell Death DifferRead more
- High Intrinsic Expression of P-glycoprotein and Breast Cancer Resistance Protein in Canine Mammary Carcinomas Regardless of Immunophenotype and Outcome. Levi M et al., 2021 Mar 2, Animals (Basel)Read more
- Photo-Oxidative Degradation Mitigated the Developmental Toxicity of Polyamide Microplastics to Zebrafish Larvae by Modulating Macrophage-Triggered Proinflammatory Responses and Apoptosis. Zou W et al., 2020 Nov 3, Environ Sci TechnolRead more
- Development of a human in vitro blood-brain tumor barrier model of diffuse intrinsic pontine glioma to better understand the chemoresistance. Deligne C et al., 2020 Jun 2, Fluids Barriers CNSRead more
- Changes in Radixin Expression and Interaction with Efflux Transporters in the Liver of Adjuvant-Induced Arthritic Rats. Kawase A et al., 2020 Feb, InflammationRead more
- Effect of heat stress on blood-brain barrier integrity in iPS cell-derived microvascular endothelial cell models. Yamaguchi T et al., 2019, PLoS OneRead more
- Organic Anion-Transporting Polypeptide 1a4 (Oatp1a4/Slco1a4) at the Blood-Arachnoid Barrier is the Major Pathway of Sulforhodamine-101 Clearance from Cerebrospinal Fluid of Rats. Yaguchi Y et al., 2019 May 6, Mol PharmRead more
- CD73 Promotes Glioblastoma Pathogenesis and Enhances Its Chemoresistance via A2B Adenosine Receptor Signaling. Yan A et al., 2019 May 29, J NeurosciRead more