More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/P Glycoprotein antibody [C219]
Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with P Glycoprotein antibody [C219] (GTX23364) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with P Glycoprotein antibody [C219] (GTX23364) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with P Glycoprotein antibody [C219] (GTX23364) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

P Glycoprotein antibody [C219]

GTX23364
GeneTex
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityBovine, Canine, Fish, Human, Mouse, Rat, Zebra Fish
TargetABCB1
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    GeneTex
  • Product Name
    P Glycoprotein antibody [C219]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:50-1:500. IHC-P: ≥1:25. FCM: . *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Flow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    C219
  • Conjugate
    Unconjugated
  • Gene ID5243
  • Target name
    ABCB1
  • Target description
    ATP binding cassette subfamily B member 1
  • Target synonyms
    ABC20, CD243, CLCS, ENPAT, GP170, MDR1, P-GP, PGY1, p-170, ATP-dependent translocase ABCB1, ATP-binding cassette, sub-family B (MDR/TAP), member 1, P-glycoprotein 1, colchicin sensitivity, doxorubicin resistance, multidrug resistance protein 1, phospholipid transporter ABCB1
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP08183
  • Protein Name
    ATP-dependent translocase ABCB1
  • Scientific Description
    The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance. The protein encoded by this gene is an ATP-dependent drug efflux pump for xenobiotic compounds with broad substrate specificity. It is responsible for decreased drug accumulation in multidrug-resistant cells and often mediates the development of resistance to anticancer drugs. This protein also functions as a transporter in the blood-brain barrier. Mutations in this gene are associated with colchicine resistance and Inflammatory bowel disease 13. Alternative splicing and the use of alternative promoters results in multiple transcript variants. [provided by RefSeq, Feb 2017]
  • Reactivity
    Bovine, Canine, Fish, Human, Mouse, Rat, Zebra Fish
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    41116161

References

  • Loss of Blood-Brain Barrier Integrity in an In Vitro Model Subjected to Intermittent Hypoxia: Is Reversion Possible with a HIF-1alpha Pathway Inhibitor?
    Read this paper
  • Blood-brain barrier-restricted translocation of Toxoplasma gondii from cortical capillaries. Olivera GC et al., 2021 Dec 8, Elife
    Read this paper
  • USP24 promotes drug resistance during cancer therapy. Wang SA et al., 2021 Sep, Cell Death Differ
    Read this paper
  • High Intrinsic Expression of P-glycoprotein and Breast Cancer Resistance Protein in Canine Mammary Carcinomas Regardless of Immunophenotype and Outcome. Levi M et al., 2021 Mar 2, Animals (Basel)
    Read this paper
  • Photo-Oxidative Degradation Mitigated the Developmental Toxicity of Polyamide Microplastics to Zebrafish Larvae by Modulating Macrophage-Triggered Proinflammatory Responses and Apoptosis. Zou W et al., 2020 Nov 3, Environ Sci Technol
    Read this paper
  • Development of a human in vitro blood-brain tumor barrier model of diffuse intrinsic pontine glioma to better understand the chemoresistance. Deligne C et al., 2020 Jun 2, Fluids Barriers CNS
    Read this paper
  • Changes in Radixin Expression and Interaction with Efflux Transporters in the Liver of Adjuvant-Induced Arthritic Rats. Kawase A et al., 2020 Feb, Inflammation
    Read this paper
  • Effect of heat stress on blood-brain barrier integrity in iPS cell-derived microvascular endothelial cell models. Yamaguchi T et al., 2019, PLoS One
    Read this paper

Datasheet

Download Datasheet

FAQ

Go to our frequently asked questions

Related products

Product group Antibodies
Absolute Antibody
Anti-P-glycoprotein [MRK16]AB00143-2.0-BT
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation
ReactivityHuman
TargetABCB1
  • SizePrice
Product group Antibodies
RayBiotech
Anti-ABCB1 Antibody144-11747
ApplicationsWestern Blot
ReactivityHuman
TargetABCB1
  • SizePrice
Product group Antibodies
Abbexa
CD243 AntibodyABX140461
ApplicationsFlow Cytometry
  • SizePrice
Product group Antibodies
Bioss
P Glycoprotein Recombinant AntibodyBSM-54782R
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
ReactivityHuman, Mouse, Rat
TargetABCB1
  • SizePrice
Figure 1. Western blot analysis of P Glycoprotein using anti-P Glycoprotein antibody (A00049-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human THP-1 whole cell lysates, Lane 2: human A375 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-P Glycoprotein antigen affinity purified polyclonal antibody (Catalog # A00049-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for P Glycoprotein at approximately 160KD. The expected band size for P Glycoprotein is at 141KD.
Product group Antibodies
Boster Bio
Anti-P Glycoprotein/ABCB1 Antibody Picoband(r)A00049-1-CARRIER-FREE
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
ReactivityHuman, Mouse, Rat
TargetABCB1
  • SizePrice
Product group Antibodies
Cusabio
MDR1 AntibodyCSB-PA001046HA01EKM
ApplicationsELISA
ReactivityParasite
  • SizePrice
Product group Antibodies
Biomatik
Abcb1 Polyclonal AntibodyCAC07057
ApplicationsImmunoFluorescence, ELISA, ImmunoHistoChemistry
TargetABCB1
  • SizePrice
Immunohistochemical staining of human kidney shows moderate positivity in apical membrane in cells in distal tubules.
Product group Antibodies
Atlas Antibodies
Anti-ABCB1 AntibodyHPA002199
ApplicationsImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman
TargetABCB1
  • SizePrice
Boiled and unboiled HepG2 membrane extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with P Glycoprotein antibody (GTX108354) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. (WCE: whole cell extract; ME: membrane extract)
Product group Antibodies
GeneTex
P Glycoprotein antibodyGTX108354
ApplicationsWestern Blot
ReactivityHuman
TargetABCB1
  • SizePrice
Unboiled HepG2 whole cell and membrane extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with P Glycoprotein antibody (GTX108370) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. (WCE: whole cell extract; ME: membrane extract)
Product group Antibodies
GeneTex
P Glycoprotein antibodyGTX108370
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
ReactivityHuman
TargetABCB1
  • SizePrice