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WB analysis of (1)NIH-3T3 and (2)MEF cell lysate using GTX20080 p19ARF antibody.
WB analysis of (1)NIH-3T3 and (2)MEF cell lysate using GTX20080 p19ARF antibody.
WB analysis of (1)NIH-3T3 and (2)MEF cell lysate using GTX20080 p19ARF antibody.

p19ARF antibody

GTX20080
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetCdkn2a
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Overview

  • Supplier
    GeneTex
  • Product Name
    p19ARF antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 2 microg/ml. ICC/IF: 1:50 - 1:100. IHC-P: 10 microg/ml. IHC-Fr: 1 microg/ml. ELISA: 1:100 - 1:2000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID12578
  • Target name
    Cdkn2a
  • Target description
    cyclin dependent kinase inhibitor 2A
  • Target synonyms
    A; alternative reading frame; Arf; ARF-; ARF-INK4a; CDK4I; cyclin-dependent kinase 4 inhibitor A; cyclin-dependent kinase inhibitor 2A; cyclin-dependent kinase inhibitor 2A (p16, inhibits CDK4); cyclin-dependent kinase inhibitor protein; INK4; Ink4a/Arf; INK4a-ARF; mitochondrial smARF; MTS; MTS1; p1; p16; p16(INK4a); p16I; p16-INK4; p16INK4a; p16-INK4a; p19; p19<A; p19<ARF>; p19ARF; Pct; Pctr1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ64364
  • Protein Name
    Tumor suppressor ARF
  • Scientific Description
    The INK4a/ARF locus encodes two unrelated tumor suppressor proteins, p16INK4a and p19ARF that restrain cell growth by modifying the functions of the retinoblastoma protein and p53, respectively. It is among the most frequently mutated tumor suppressor loci in human cancer. Both p16INK4a and p19ARF act as cell proliferation inhibitors. The ARF gene, p19ARF in mouse and p14ARF in human, has become an important player in cell cycle regulation. In mice, tumor suppressor effects appear to be mediated by interactions between p19ARF and the p53 tumor-suppressor protein. p19ARF counters uncontrolled proliferation and oncogenic signals in p53-dependent pathways. Proteins encoded by the INK4a/ARF locus also play a role in Abelson virus transformation. Both p16INK4a and p19ARF are expressed in many cells as they emerge from the apoptotic crisis that characterizes the transformation process. Expression of p19ARF but not p16INK4a induces apoptosis in Ab-MLV-transformed pre-B cells. INK4a/ARF expression correlates with or precedes the emergence of cells expressing mutant p53. p19ARF is an important part in cellular defense against transforming signals from the Abl oncoprotein, providing direct evidence that the p19ARF-p53 regulatory loop plays an important role in lymphoma induction.
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Loss of fragile WWOX gene leads to senescence escape and genome instability.
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  • High glucose induces bone marrow-derived mesenchymal stem cell senescence by upregulating autophagy. Chang TC et al., 2015, PLoS One
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  • Inactivation of p53 is insufficient to allow B cells and B-cell lymphomas to survive without Dicer. Adams CM et al., 2014 Jul 15, Cancer Res
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  • The p53 Codon 72 Polymorphism Modifies the Cellular Response to Inflammatory Challenge in the Liver. Leu JI et al., 2013, J Liver
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  • Suppression of Ras/Mapk pathway signaling inhibits Myc-induced lymphomagenesis. Gramling MW et al., 2012 Jul, Cell Death Differ
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  • Egr1 mediates p53-independent c-Myc-induced apoptosis via a noncanonical ARF-dependent transcriptional mechanism. Boone DN et al., 2011 Jan 11, Proc Natl Acad Sci U S A
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  • MicroRNA biogenesis is required for Myc-induced B-cell lymphoma development and survival. Arrate MP et al., 2010 Jul 15, Cancer Res
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  • The ARF tumor suppressor can promote the progression of some tumors. Humbey O et al., 2008 Dec 1, Cancer Res
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  • Foxm1b transcription factor is essential for development of hepatocellular carcinomas and is negatively regulated by the p19ARF tumor suppressor. Kalinichenko VV et al., 2004 Apr 1, Genes Dev
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