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The image on the left is immunohistochemistry of paraffin-embedded Human ovarian cancer tissue using CSB-PA576070(P2RY6 Antibody) at dilution 1/40, on the right is treated with synthetic peptide. (Original magnification: x200)
The image on the left is immunohistochemistry of paraffin-embedded Human ovarian cancer tissue using CSB-PA576070(P2RY6 Antibody) at dilution 1/40, on the right is treated with synthetic peptide. (Original magnification: x200)
The image on the left is immunohistochemistry of paraffin-embedded Human ovarian cancer tissue using CSB-PA576070(P2RY6 Antibody) at dilution 1/40, on the right is treated with synthetic peptide. (Original magnification: x200)

P2RY6 Antibody

CSB-PA576070
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetP2RY6
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Overview

  • Supplier
    Cusabio
  • Product Name
    P2RY6 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5031
  • Target name
    P2RY6
  • Target description
    pyrimidinergic receptor P2Y6
  • Target synonyms
    G-coupled nucleotide receptor; P2 purinoceptor; P2Y purinoceptor 6; P2Y6; P2Y6 receptor; pyrimidinergic receptor P2Y, G-protein coupled, 6
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ15077
  • Protein Name
    P2Y purinoceptor 6
  • Scientific Description
    The product of this gene belongs to the family of G-protein coupled receptors. This family has several receptor subtypes with different pharmacological selectivity, which overlaps in some cases, for various adenosine and uridine nucleotides. This receptor is responsive to UDP, partially responsive to UTP and ADP, and not responsive to ATP. Four transcript variants encoding the same isoform have been identified for this gene
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of P2Y6/P2RY6 using anti-P2Y6/P2RY6 antibody (A10087-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hacat whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-P2Y6/P2RY6 antigen affinity purified polyclonal antibody (Catalog # A10087-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for P2Y6/P2RY6 at approximately 45 kDa. The expected band size for P2Y6/P2RY6 is at 36 kDa.
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