Non-transfected (–) and transfected (+) boiled and unboiled 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with P2Y1 antibody [HL2702] (GTX639453) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
![Boiled and unboiled LoVo whole cell and membrane extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with P2Y1 antibody [HL2702] (GTX639453) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](https://www.genetex.com/upload/website/prouct_img/normal/GTX639453/GTX639453_45306_20240202_WB_Fraction_24021917_207.webp "Boiled and unboiled LoVo whole cell and membrane extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with P2Y1 antibody [HL2702] (GTX639453) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
![Boiled and unboiled LoVo whole cell and membrane extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with P2Y1 antibody [HL2702] (GTX639453) diluted at 1:1000 and competitor's antibody (competitor) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. *The competitor is not affiliated with GeneTex and does not endorse this product.](https://www.genetex.com/upload/website/prouct_img/normal/GTX639453/GTX639453_45306_20240223_WB_Fraction_competitor_watermark_24022619_183.webp "Boiled and unboiled LoVo whole cell and membrane extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with P2Y1 antibody [HL2702] (GTX639453) diluted at 1:1000 and competitor's antibody (competitor) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. *The competitor is not affiliated with GeneTex and does not endorse this product.")
![Non-transfected (–) and transfected (+) boiled and unboiled 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with P2Y1 antibody [HL2702] (GTX639453) diluted at 1:5000 and competitor's antibody (competitor) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. *The competitor is not affiliated with GeneTex and does not endorse this product.](https://www.genetex.com/upload/website/prouct_img/normal/GTX639453/GTX639453_45306_20240223_WB_B_competitor_watermark_24022619_781.webp "Non-transfected (–) and transfected (+) boiled and unboiled 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with P2Y1 antibody [HL2702] (GTX639453) diluted at 1:5000 and competitor's antibody (competitor) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. *The competitor is not affiliated with GeneTex and does not endorse this product.")
![P2Y1 antibody [HL2702] detects P2Y1 protein by immunohistochemical analysis. Sample: Paraffin-embedded human pancreas. P2Y1 stained by P2Y1 antibody [HL2702] (GTX639453) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX639453/GTX639453_T-45236_20250418_IHC-P_25050623_639.webp "P2Y1 antibody [HL2702] detects P2Y1 protein by immunohistochemical analysis. Sample: Paraffin-embedded human pancreas. P2Y1 stained by P2Y1 antibody [HL2702] (GTX639453) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Unboiled non-transfected (–) and transfected (+) 293T and Expi293 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with P2Y1 antibody [HL2702] (GTX639453) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX639453/GTX639453_45306_20250502_WB_multiple_B_25051420_485.webp "Unboiled non-transfected (–) and transfected (+) 293T and Expi293 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with P2Y1 antibody [HL2702] (GTX639453) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
Non-transfected (–) and transfected (+) boiled and unboiled 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with P2Y1 antibody [HL2702] (GTX639453) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
P2Y1 antibody [HL2702]
GTX639453
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman
TargetP2RY1
Overview
- SupplierGeneTex
- Product NameP2Y1 antibody [HL2702]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:5000-1:50000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsWestern Blot
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDHL2702
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID5028
- Target nameP2RY1
- Target descriptionpurinergic receptor P2Y1
- Target synonymsP2Y1, SARCC, P2Y purinoceptor 1, ADP receptor, ATP receptor, P2 purinoceptor subtype Y1, platelet ADP receptor, purinergic receptor P2Y, G-protein coupled, 1, suppressing androgen receptor in renal cell carcinoma
- HostRabbit
- IsotypeIgG
- Protein IDP47900
- Protein NameP2Y purinoceptor 1
- Scientific DescriptionThe product of this gene belongs to the family of G-protein coupled receptors. This family has several receptor subtypes with different pharmacological selectivity, which overlaps in some cases, for various adenosine and uridine nucleotides. This receptor functions as a receptor for extracellular ATP and ADP. In platelets binding to ADP leads to mobilization of intracellular calcium ions via activation of phospholipase C, a change in platelet shape, and probably to platelet aggregation. [provided by RefSeq, Jul 2008]
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
Datasheet
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