Non-transfected (–) and transfected (+) boiled and unboiled 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with P2Y2 antibody [HL2704] (GTX639458) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
![Boiled and unboiled HL-60 whole cell and membrane extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with P2Y2 antibody [HL2704] (GTX639458) diluted at 1:1000 and competitor's antibody (competitor) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. *The competitor is not affiliated with GeneTex and does not endorse this product.](https://www.genetex.com/upload/website/prouct_img/normal/GTX639458/GTX639458_45306_20240419_WB_Fraction_competitor_watermark_24041523_603.webp "Boiled and unboiled HL-60 whole cell and membrane extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with P2Y2 antibody [HL2704] (GTX639458) diluted at 1:1000 and competitor's antibody (competitor) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. *The competitor is not affiliated with GeneTex and does not endorse this product.")
![Non-transfected (–) and transfected (+) boiled and unboiled 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with P2Y2 antibody [HL2704] (GTX639458) diluted at 1:5000 and competitor's antibody (competitor) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. *The competitor is not affiliated with GeneTex and does not endorse this product.](https://www.genetex.com/upload/website/prouct_img/normal/GTX639458/GTX639458_45306_20240419_WB_B_competitor_watermark_24041523_623.webp "Non-transfected (–) and transfected (+) boiled and unboiled 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with P2Y2 antibody [HL2704] (GTX639458) diluted at 1:5000 and competitor's antibody (competitor) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. *The competitor is not affiliated with GeneTex and does not endorse this product.")
![P2Y2 antibody [HL2704] detects P2Y2 protein at cell membrane by immunofluorescent analysis. Sample: THP-1 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: P2Y2 stained by P2Y2 antibody [HL2704] (GTX639458) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).](https://www.genetex.com/upload/website/prouct_img/normal/GTX639458/GTX639458_45306_20240426_ICC_IF_24051400_322.webp "P2Y2 antibody [HL2704] detects P2Y2 protein at cell membrane by immunofluorescent analysis. Sample: THP-1 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: P2Y2 stained by P2Y2 antibody [HL2704] (GTX639458) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).")
![Boiled and unboiled HL-60 whole cell and membrane extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with P2Y2 antibody [HL2704] (GTX639458) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.(WCE: whole cell extract; ME: membrane extract)](https://www.genetex.com/upload/website/prouct_img/normal/GTX639458/GTX639458_45390_20240503_WB_Fraction_24090423_788.webp "Boiled and unboiled HL-60 whole cell and membrane extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with P2Y2 antibody [HL2704] (GTX639458) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.(WCE: whole cell extract; ME: membrane extract)")
![Non-transfected (–) and transfected (+) unboiled 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with P2Y2 antibody [HL2704] (GTX639458) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX639458/GTX639458_45558_20250502_WB_multiple_B_25100823_237.webp "Non-transfected (–) and transfected (+) unboiled 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with P2Y2 antibody [HL2704] (GTX639458) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![Untreated (–) and treated (+) unboiled HL-60 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with P2Y2 antibody [HL2704] (GTX639458) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX639458/GTX639458_45558_20250214_WB_treatment_PNGaseF_25100823_754.webp "Untreated (–) and treated (+) unboiled HL-60 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with P2Y2 antibody [HL2704] (GTX639458) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
Non-transfected (–) and transfected (+) boiled and unboiled 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with P2Y2 antibody [HL2704] (GTX639458) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
P2Y2 antibody [HL2704]
GTX639458
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetP2RY2
Overview
- SupplierGeneTex
- Product NameP2Y2 antibody [HL2704]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDHL2704
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID5029
- Target nameP2RY2
- Target descriptionpurinergic receptor P2Y2
- Target synonymsHP2U, P2RU1, P2U, P2U1, P2UR, P2Y2, P2Y2R, P2Y purinoceptor 2, ATP receptor, P2U nucleotide receptor, P2U purinoceptor 1, P2U receptor 1, purinergic receptor P2Y, G-protein coupled, 2, purinoceptor P2Y2
- HostRabbit
- IsotypeIgG
- Protein IDP41231
- Protein NameP2Y purinoceptor 2
- Scientific DescriptionThe product of this gene belongs to the family of P2 receptors, which is activated by extracellular nucleotides and subdivided into P2X ligand-gated ion channels and P2Y G-protein coupled receptors. This family has several receptor subtypes with different pharmacological selectivity, which overlaps in some cases, for various adenosine and uridine nucleotides. This receptor, found on many cell types, is activated by ATP and UTP and is reported to be overexpressed on some cancer cell types. It is involved in many cellular functions, such as proliferation, apoptosis and inflammation. Three transcript variants encoding the same protein have been identified for this gene. [provided by RefSeq, Mar 2013]
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC41116161
Datasheet
Related products
Product group Antibodies
P2RY2 AntibodyCSB-PA079605
ApplicationsWestern Blot, ELISA
ReactivityHuman
TargetP2RY2
- SizePrice
Product group Antibodies
Anti-P2RY2 Antibody144-05779
ApplicationsImmunoFluorescence, Western Blot
ReactivityHuman, Mouse
TargetP2RY2
- SizePrice
Product group Antibodies
Anti-P2RY2 AntibodyA31047
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse
- SizePrice
Product group Antibodies
ApplicationsImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
TargetP2RY2
- SizePrice
Product group Antibodies
P2RY2 / P2Y2 Antibody (aa353-366)LS-C402497
ApplicationsWestern Blot, ELISA
ReactivityHuman
TargetP2RY2
- SizePrice
Product group Antibodies
References
P2Y2 Polyclonal AntibodyBS-4180R
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
ReactivityCanine, Equine, Human, Mouse, Rabbit, Rat
TargetP2RY2
- SizePrice
![P2Y2 antibody [N3C3] detects P2Y2 protein at cell membrane by immunofluorescent analysis. Sample: K562 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: P2Y2 stained by P2Y2 antibody [N3C3] (GTX114609) diluted at 1:500. Blue: Hoechst 33342 staining.](https://www.genetex.com/upload/website/prouct_img/normal/GTX114609/GTX114609_44321_20220415_ICC_IF_w_23060518_720.webp)
Product group Antibodies
P2Y2 antibody [N3C3]GTX114609
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
ReactivityHuman
TargetP2RY2
- SizePrice
