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p38 alpha/MAPK14 Antibody (YA3534, Rabbit)

HY-P80996
MedChem Express
TargetMAPK14
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    p38 alpha/MAPK14 Antibody (YA3534, Rabbit)
  • Delivery Days Customer
    10
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID1432
  • Target name
    MAPK14
  • Target description
    mitogen-activated protein kinase 14
  • Target synonyms
    CSBP, CSBP1, CSBP2, CSPB1, EXIP, Mxi2, PRKM14, PRKM15, RK, SAPK2A, p38, p38ALPHA, mitogen-activated protein kinase 14, CSAID-binding protein, MAP kinase 14, MAP kinase Mxi2, MAP kinase p38 alpha, MAX-interacting protein 2, cytokine suppressive anti-inflammatory drug binding protein, mitogen-activated protein kinase p38 alpha, p38 MAP kinase, p38 mitogen activated protein kinase, p38alpha Exip, stress-activated protein kinase 2A
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    p38 alpha/MAPK14 (YA3534) is an unconjugated, approximately 41 kDa, anti-p38 MAPK monoclonal antibody. p38 alpha/MAPK14 (YA3534) can be used for: WB, IF-Cell, IF-Tissue expriments in human, mouse, rat background without labeling.
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of P38 Alpha/MAPK14 using anti-P38 Alpha/MAPK14 antibody (A00176-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human SW620 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: rat lung tissue lysates, Lane 5: rat heart tissue lysates, Lane 6: mouse lung tissue lysates, Lane 7: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-P38 Alpha/MAPK14 antigen affinity purified polyclonal antibody (Catalog # A00176-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for P38 Alpha/MAPK14 at approximately 41 kDa. The expected band size for P38 Alpha/MAPK14 is at 41 kDa.
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