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Western Blot Positive WB detected in: Mouse lung tissue, Mouse kidney tissue All lanes: LEPREL1 antibody at 3ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 81, 61 kDa Observed band size: 81, 61 kDa
Western Blot Positive WB detected in: Mouse lung tissue, Mouse kidney tissue All lanes: LEPREL1 antibody at 3ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 81, 61 kDa Observed band size: 81, 61 kDa
Western Blot Positive WB detected in: Mouse lung tissue, Mouse kidney tissue All lanes: LEPREL1 antibody at 3ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 81, 61 kDa Observed band size: 81, 61 kDa

P3H2 Antibody

CSB-PA815555HA01HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetP3H2
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Overview

  • Supplier
    Cusabio
  • Product Name
    P3H2 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID55214
  • Target name
    P3H2
  • Target description
    prolyl 3-hydroxylase 2
  • Target synonyms
    LEPREL1, MCVD, MLAT4, prolyl 3-hydroxylase 2, leprecan-like 1, myxoid liposarcoma-associated protein 4, procollagen-proline 3-dioxygenase 2, prolyl 3-hydroxylase 3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8IVL5
  • Protein Name
    Prolyl 3-hydroxylase 2
  • Scientific Description
    Shows prolyl 3-hydroxylase activity catalyzing the post-translational formation of 3-hydroxyproline in -Xaa-Pro-Gly-sequences in collagens, especially types II, IV and V.
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of P3H2 using anti-P3H2 antibody (A09235-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human RT4 whole cell lysates, Lane 3: human SIHA whole cell lysates, Lane 4: human A431 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-P3H2 antigen affinity purified polyclonal antibody (Catalog # A09235-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for P3H2 at approximately 81 kDa. The expected band size for P3H2 is at 81,60 kDa.
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