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PABPC4 Rabbit pAb

A5948
ABclonal
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetPABPC4
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Overview

  • Supplier
    ABclonal
  • Product Name
    PABPC4 Rabbit pAb
  • Delivery Days Customer
    12
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB,IHC,IF/ICC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID8761
  • Target name
    PABPC4
  • Target description
    poly(A) binding protein cytoplasmic 4
  • Target synonyms
    APP-1, APP1, PABP4, iPABP, polyadenylate-binding protein 4, PABP-4, activated-platelet protein 1, inducible poly(A)-binding protein, poly(A) binding protein, cytoplasmic 4 (inducible form), poly(A)-binding protein 4
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ13310
  • Protein Name
    Polyadenylate-binding protein 4
  • Reactivity Supplier Note
    Human,Mouse,Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of APP-1/PABPC4 using anti-APP-1/PABPC4 antibody (A07960-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: human THP-1 whole cell lysates, Lane 5: human RT4 whole cell lysates, Lane 6: human A431 whole cell lysates, Lane 7: human Daudi whole cell lysates, Lane 8: rat testis tissue lysates, Lane 9: rat heart tissue lysates, Lane 10: rat brain tissue lysates, Lane 11: rat L6 whole cell lysates, Lane 12: mouse heart tissue lysates, Lane 13: mouse brain tissue lysates, Lane 14: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-APP-1/PABPC4 antigen affinity purified polyclonal antibody (Catalog # A07960-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for APP-1/PABPC4 at approximately 75 kDa. The expected band size for APP-1/PABPC4 is at 71 kDa.
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