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PADI3 Antibody, Biotin conjugated

CSB-PA891552LD01HU
Cusabio
ApplicationsELISA
Product group Antibodies
ReactivityHuman
TargetPADI3
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Overview

  • Supplier
    Cusabio
  • Product Name
    PADI3 Antibody, Biotin conjugated
  • Delivery Days Customer
    20
  • Applications
    ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Biotin
  • Gene ID51702
  • Target name
    PADI3
  • Target description
    peptidyl arginine deiminase 3
  • Target synonyms
    PAD3, PDI3, UHS1, protein-arginine deiminase type-3, peptidyl arginine deiminase, type III, peptidylarginine deiminase III, protein-arginine deiminase type III
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9ULW8
  • Protein Name
    Protein-arginine deiminase type-3
  • Scientific Description
    Catalyzes the deimination of arginine residues of proteins.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of PADI3 using anti-PADI3 antibody (A11754-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT4 whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: rat C6 whole cell lysates, Lane 4: mouse kidney tissue lysates, Lane 5: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PADI3 antigen affinity purified polyclonal antibody (Catalog # A11754-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PADI3 at approximately 75 kDa. The expected band size for PADI3 is at 75 kDa.
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