Bio-Connect
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with PAFAH Polyclonal Antibody, Unconjugated (bs-1451R) at 1:400 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with PAFAH Polyclonal Antibody, Unconjugated (bs-1451R) at 1:400 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with PAFAH Polyclonal Antibody, Unconjugated (bs-1451R) at 1:400 overnight at 4°C, DAB staining.

PLA2G7 Polyclonal Antibody

BS-1451R
Bioss Antibodies
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetPLA2G7
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request

Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    PAFAH POLYCLONAL ANTIBODY
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID7941
  • Target name
    PLA2G7
  • Target description
    phospholipase A2 group VII
  • Target synonyms
    1-alkyl-2-acetylglycerophosphocholine esterase; 2-acetyl-1-alkylglycerophosphocholine esterase; group-VIIA phospholipase A2; gVIIA-PLA2; LDL-associated phospholipase A2; LDL-PLA(2); LDL-PLA2; lipoprotein-associated phospholipase A2; LP-PLA2; PAF 2-acylhydrolase; PAF acetylhydrolase; PAFAD; PAFAH; phospholipase A2, group VII (platelet-activating factor acetylhydrolase, plasma); platelet-activating factor acetylhydrolase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ13093
  • Protein Name
    Platelet-activating factor acetylhydrolase
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Apolipoprotein CIII regulates lipoprotein-associated phospholipase A2 expression via the MAPK and NFkappaB pathways. Han X et al., 2015 Apr 2, Biol Open
    Read more

Related products

Product group Antibodies
Biomatik
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
TargetPLA2G7
  • SizePrice
Product group Antibodies
Assay Genie
TargetPLA2G7
  • SizePrice
Anti-PLA2G7 Antibody
Product group Antibodies
Antibodies.com
ApplicationsWestern Blot
  • SizePrice
Product group Antibodies
RayBiotech
ApplicationsWestern Blot
TargetPLA2G7
  • SizePrice
Product group Antibodies
MedChem Express
TargetPLA2G7
  • SizePrice
Immunohistochemistry of paraffin-embedded human colon cancer using CSB-PA08917A0Rb at dilution of 1:100
Product group Antibodies
Cusabio
PLA2G7 AntibodyCSB-PA08917A0RB
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman
TargetPLA2G7
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
ApplicationsWestern Blot
TargetPLA2G7
  • SizePrice
Figure 1. Western blot analysis of PAFAH using anti-PAFAH antibody (A02164-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysate, Lane 2: human SW620 whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PAFAH antigen affinity purified polyclonal antibody (Catalog # A02164-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PAFAH at approximately 50KD. The expected band size for PAFAH is at 50KD.
Product group Antibodies
Boster Bio
ApplicationsWestern Blot, ELISA
TargetPLA2G7
  • SizePrice
Product group Antibodies
Everest Biotech
ApplicationsELISA
TargetPLA2G7
  • SizePrice
Product group Antibodies
GeneTex
ApplicationsWestern Blot
TargetPLA2G7
  • SizePrice