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Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with PAFAH Polyclonal Antibody, Unconjugated (bs-1451R) at 1:400 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with PAFAH Polyclonal Antibody, Unconjugated (bs-1451R) at 1:400 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with PAFAH Polyclonal Antibody, Unconjugated (bs-1451R) at 1:400 overnight at 4°C, DAB staining.

PLA2G7 Polyclonal Antibody

BS-1451R
Bioss
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetPLA2G7
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Overview

  • Supplier
    Bioss
  • Product Name
    PLA2G7 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID7941
  • Target name
    PLA2G7
  • Target description
    phospholipase A2 group VII
  • Target synonyms
    LDL-PLA2, LP-PLA2, PAFAD, PAFAH, platelet-activating factor acetylhydrolase, 1-alkyl-2-acetylglycerophosphocholine esterase, 2-acetyl-1-alkylglycerophosphocholine esterase, LDL-PLA(2), LDL-associated phospholipase A2, PAF 2-acylhydrolase, PAF acetylhydrolase, gVIIA-PLA2, group-VIIA phospholipase A2, lipoprotein-associated phospholipase A2, phospholipase A2, group VII (platelet-activating factor acetylhydrolase, plasma)
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ13093
  • Protein Name
    Platelet-activating factor acetylhydrolase
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Han X, Wang T, Zhang J, et al. Apolipoprotein CIII regulates lipoprotein-associated phospholipase A2 expression via the MAPK and NFκB pathways. Biol Open. 2015,4(5):661-5. doi: 10.1242/bio.201410900
    Read this paper

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Figure 1. Western blot analysis of PAFAH using anti-PAFAH antibody (A02164-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysate, Lane 2: human SW620 whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PAFAH antigen affinity purified polyclonal antibody (Catalog # A02164-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PAFAH at approximately 50KD. The expected band size for PAFAH is at 50KD.
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