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ProductsBack/PAI-RBP1 / SERBP1 Antibody

PAI-RBP1 / SERBP1 Antibody

LS-C749849
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetSERBP1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    PAI-RBP1 / SERBP1 Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 42kDa/43kDa/44kDa.
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:500 - 1:2000) The predicted MW is 42kDa/43kDa/44kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID26135
  • Target name
    SERBP1
  • Target description
    SERPINE1 mRNA binding protein 1
  • Target synonyms
    CGI-55, CHD3IP, HABP4L, Hero45, PAI-RBP1, PAIRBP1, SERPINE1 mRNA-binding protein 1, PAI-1 mRNA binding protein, PAI1 RNA-binding protein 1, chromodomain helicase DNA binding protein 3 interacting protein, plasminogen activator inhibitor 1 RNA-binding protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SERBP1 using anti-SERBP1 antibody (A04755-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human LNCAP whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human Daudi whole cell lysates, Lane 4: human HEL whole cell lysates, Lane 5: rat kidney tissue lysates, Lane 6: mouse skeletal muscle tissue lysates, Lane 7: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SERBP1 antigen affinity purified polyclonal antibody (Catalog # A04755-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SERBP1 at approximately 55 kDa(human) and 50 kDa(mouse, rat). The expected band size for SERBP1 is at 45 kDa.
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