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Western blot All lanes: PAICSantibody at 3.98ug/ml Lane 1: Hela whole cell lysate Lane 2: Raji whole cell lysate Lane 3: 293T whole cell lysate Lane 4: NIH/3T3 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 47 kDa Observed band size: 47 kDa
Western blot All lanes: PAICSantibody at 3.98ug/ml Lane 1: Hela whole cell lysate Lane 2: Raji whole cell lysate Lane 3: 293T whole cell lysate Lane 4: NIH/3T3 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 47 kDa Observed band size: 47 kDa
Western blot All lanes: PAICSantibody at 3.98ug/ml Lane 1: Hela whole cell lysate Lane 2: Raji whole cell lysate Lane 3: 293T whole cell lysate Lane 4: NIH/3T3 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 47 kDa Observed band size: 47 kDa

PAICS Antibody

CSB-PA017397ESR1HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetPAICS
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Overview

  • Supplier
    Cusabio
  • Product Name
    PAICS Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID10606
  • Target name
    PAICS
  • Target description
    phosphoribosylaminoimidazole carboxylase and phosphoribosylaminoimidazolesuccinocarboxamide synthase
  • Target synonyms
    ADE2; ADE2H1; AIR carboxylase; AIRC; multifunctional protein ADE2; multifunctional protein ADE2H1; PAIS; phosphoribosylaminoimidazole carboxylase, phosphoribosylaminoimidazole succinocarboxamide synthetase; phosphoribosylaminoimidazole carboxylase; phosphoribosylaminoimidazolesuccinocarboxamide synthase; SAICAR synthetase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP22234
  • Protein Name
    Multifunctional protein ADE2
  • Scientific Description
    cytosol, extracellular exosome, membrane, identical protein binding, phosphoribosylaminoimidazole carboxylase activity, phosphoribosylaminoimidazolesuccinocarboxamide synthase activity, de novo IMP biosynthetic process, adenine biosynthetic process, purine nucleobase biosynthetic process, purine ribonucleoside monophosphate biosynthetic process
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PAICS using anti-PAICS antibody (A07031-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human Raji whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PAICS antigen affinity purified polyclonal antibody (Catalog # A07031-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PAICS at approximately 47 kDa. The expected band size for PAICS is at 47 kDa.
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