Untreated (-) and treated (+) HeLa whole cell extracts (30 microg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PAK4 (phospho Ser474) / PAK5 (phospho Ser602) / PAK6 (phospho Ser560) antibody [GT1344] (GTX03256) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
![WB analysis of NIH-3T3 cells were treated by PMA/TPA (200 nM) at 37oC for 30 minutes after serum-starvation overnight using GTX03256 PAK4 (phospho Ser474) / PAK5 (phospho Ser602) / PAK6 (phospho Ser560) antibody [GT1344]. Dilution : 1:1000 Loading : 25microg per lane](https://www.genetex.com/upload/website/prouct_img/normal/GTX03256/GTX03256_92_WB_w_23053123_302.webp "WB analysis of NIH-3T3 cells were treated by PMA/TPA (200 nM) at 37oC for 30 minutes after serum-starvation overnight using GTX03256 PAK4 (phospho Ser474) / PAK5 (phospho Ser602) / PAK6 (phospho Ser560) antibody [GT1344]. Dilution : 1:1000 Loading : 25microg per lane")
![WB analysis of C6 cells were treated by PMA/TPA (200 nM) at 37oC for 30 minutes after serum-starvation overnight using GTX03256 PAK4 (phospho Ser474) / PAK5 (phospho Ser602) / PAK6 (phospho Ser560) antibody [GT1344]. Dilution : 1:1000 Loading : 25microg per lane](https://www.genetex.com/upload/website/prouct_img/normal/GTX03256/GTX03256_93_WB_w_23053123_171.webp "WB analysis of C6 cells were treated by PMA/TPA (200 nM) at 37oC for 30 minutes after serum-starvation overnight using GTX03256 PAK4 (phospho Ser474) / PAK5 (phospho Ser602) / PAK6 (phospho Ser560) antibody [GT1344]. Dilution : 1:1000 Loading : 25microg per lane")
Untreated (-) and treated (+) HeLa whole cell extracts (30 microg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PAK4 (phospho Ser474) / PAK5 (phospho Ser602) / PAK6 (phospho Ser560) antibody [GT1344] (GTX03256) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
PAK4 (phospho Ser474) / PAK5 (phospho Ser602) / PAK6 (phospho Ser560) antibody [GT1344]
GTX03256
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetPAK4
Overview
- SupplierGeneTex
- Product NamePAK4 (phospho Ser474) / PAK5 (phospho Ser602) / PAK6 (phospho Ser560) antibody [GT1344] - Orthogonal Validated
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500 - 1:2000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsWestern Blot
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDGT1344
- Concentration1.65 mg/ml
- ConjugateUnconjugated
- Gene ID10298
- Target namePAK4
- Target descriptionp21 (RAC1) activated kinase 4
- Target synonymsp21 protein (Cdc42/Rac)-activated kinase 4; p21(CDKN1A)-activated kinase 4; protein kinase related to S. cerevisiae STE20, effector for Cdc42Hs; serine/threonine-protein kinase PAK 4
- HostRabbit
- IsotypeIgG
- Protein IDO96013
- Protein NameSerine/threonine-protein kinase PAK 4
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203