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Western Blot analysis of 3T3 cells using PAK5 Polyclonal Antibody
Western Blot analysis of 3T3 cells using PAK5 Polyclonal Antibody
Western Blot analysis of 3T3 cells using PAK5 Polyclonal Antibody

PAK7 Antibody

CSB-PA003702
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetPAK5
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Overview

  • Supplier
    Cusabio
  • Product Name
    PAK7 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID57144
  • Target name
    PAK5
  • Target description
    p21 (RAC1) activated kinase 5
  • Target synonyms
    p21 (RAC1) activated kinase 7; p21 protein (Cdc42/Rac)-activated kinase 7; p21(CDKN1A)-activated kinase 7; p21-activated kinase 5; p21-activated kinase 7; p21CDKN1A-activated kinase 7; PAK-5; PAK7; PAK-7; protein kinase PAK5; serine/threonine-protein kinase PAK 5; serine/threonine-protein kinase PAK 7; serine/threonine-protein kinase PAK7
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9P286
  • Protein Name
    Serine/threonine-protein kinase PAK 5
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PAK5 using anti-PAK5 antibody (PB9733). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: rat brain tissue lysates, Lane 3: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PAK5 antigen affinity purified polyclonal antibody (Catalog # PB9733) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PAK5 at approximately 81 kDa. The expected band size for PAK5 is at 81 kDa.
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