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Western Blot Positive WB detected in: Mouse kidney tissue All lanes: PALM antibody at 2.7ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 43, 38 kDa Observed band size: 43 kDa
Western Blot Positive WB detected in: Mouse kidney tissue All lanes: PALM antibody at 2.7ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 43, 38 kDa Observed band size: 43 kDa
Western Blot Positive WB detected in: Mouse kidney tissue All lanes: PALM antibody at 2.7ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 43, 38 kDa Observed band size: 43 kDa

PALM Antibody

CSB-PA017413HA01HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetPALM
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Overview

  • Supplier
    Cusabio
  • Product Name
    PALM Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5064
  • Target name
    PALM
  • Target description
    paralemmin
  • Target synonyms
    PALM1; paralemmin-1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO75781
  • Protein Name
    Paralemmin-1
  • Scientific Description
    Involved in plasma membrane dynamics and cell process formation. Isoform 1 and isoform 2 are necessary for axonal and dendritic filopodia induction, for dendritic spine maturation and synapse formation in a palmitoylation-dependent manner.
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of PALM using anti-PALM antibody (A00265-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U20S whole cell lysates, Lane 2: rat brain tissue lysates, Lane 3: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PALM antigen affinity purified polyclonal antibody (Catalog # A00265-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PALM at approximately 37 kDa. The expected band size for PALM is at 42 kDa.
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Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in distal tubules.
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