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PALMD Antibody (aa325-375)

LS-C763297
LifeSpan BioSciences
ApplicationsImmunoPrecipitation, Western Blot
Product group Antibodies
TargetPALMD
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    PALMD Antibody (aa325-375)
  • Delivery Days Customer
    23
  • Application Supplier Note
    Immunoprecipitation Range: 50-100 microl/mg lysate
  • Applications
    ImmunoPrecipitation, Western Blot
  • Applications Supplier
    IP, WB (1:1000) Immunoprecipitation Range: 50-100 µl/mg lysate
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID54873
  • Target name
    PALMD
  • Target description
    palmdelphin
  • Target synonyms
    C1orf11, PALML, palmdelphin, paralemmin-like protein, paralemnin-like protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PALMD using anti-PALMD antibody (A11148-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PALMD antigen affinity purified polyclonal antibody (Catalog # A11148-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PALMD at approximately 68 kDa. The expected band size for PALMD is at 63 kDa.
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