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Western Blot Positive WB detected in: MCF-7 whole cell lysate All lanes: PANX1 antibody at 3.2microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 49, 48 kDa Observed band size: 49 kDa
Western Blot Positive WB detected in: MCF-7 whole cell lysate All lanes: PANX1 antibody at 3.2microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 49, 48 kDa Observed band size: 49 kDa
Western Blot Positive WB detected in: MCF-7 whole cell lysate All lanes: PANX1 antibody at 3.2microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 49, 48 kDa Observed band size: 49 kDa

PANX1 Antibody

CSB-PA857017LA01HU
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetPANX1
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Overview

  • Supplier
    Cusabio
  • Product Name
    PANX1 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID24145
  • Target name
    PANX1
  • Target description
    pannexin 1
  • Target synonyms
    innexin; MRS1; OOMD7; pannexin-1; PX1; UNQ2529
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ96RD7
  • Protein Name
    Pannexin-1
  • Scientific Description
    Structural component of the gap junctions and the hemichannels. May play a role as a Ca(2+)-leak channel to regulate ER Ca(2+) homeostasis.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Pannexin 1/PANX1 using anti-Pannexin 1/PANX1 antibody (A00915-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: rat C6 whole cell lysates, Lane 6: mouse brain tissue lysates, Lane 7: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Pannexin 1/PANX1 antigen affinity purified polyclonal antibody (Catalog # A00915-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Pannexin 1/PANX1 at approximately 48 kDa. The expected band size for Pannexin 1/PANX1 is at 48 kDa.
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