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Western Blot Positive WB detected in: HepG2 whole cell lysate, K562 whole cell lysate, Rat liver tissue, Mouse liver tissue All lanes: PANX2 antibody at 4.2microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 75, 71, 56 kDa Observed band size: 75 kDa
Western Blot Positive WB detected in: HepG2 whole cell lysate, K562 whole cell lysate, Rat liver tissue, Mouse liver tissue All lanes: PANX2 antibody at 4.2microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 75, 71, 56 kDa Observed band size: 75 kDa
Western Blot Positive WB detected in: HepG2 whole cell lysate, K562 whole cell lysate, Rat liver tissue, Mouse liver tissue All lanes: PANX2 antibody at 4.2microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 75, 71, 56 kDa Observed band size: 75 kDa

PANX2 Antibody

CSB-PA839405LA01HU
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetPANX2
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Overview

  • Supplier
    Cusabio
  • Product Name
    PANX2 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID56666
  • Target name
    PANX2
  • Target description
    pannexin 2
  • Target synonyms
    PX2, hPANX2, pannexin-2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ96RD6
  • Protein Name
    Pannexin-2
  • Scientific Description
    Structural component of the gap junctions and the hemichannels.
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of PANX2 using anti-PANX2 antibody (A08860-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: humna SW579 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PANX2 antigen affinity purified polyclonal antibody (Catalog # A08860-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PANX2 at approximately 74KD. The expected band size for PANX2 is at 74KD.
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