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Western Blot Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate All lanes: PANX3 antibody at 4microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 45 kDa Observed band size: 45 kDa
Western Blot Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate All lanes: PANX3 antibody at 4microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 45 kDa Observed band size: 45 kDa
Western Blot Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate All lanes: PANX3 antibody at 4microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 45 kDa Observed band size: 45 kDa

PANX3 Antibody

CSB-PA839402LA01HU
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetPANX3
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Overview

  • Supplier
    Cusabio
  • Product Name
    PANX3 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID116337
  • Target name
    PANX3
  • Target description
    pannexin 3
  • Target synonyms
    PX3, pannexin-3, gap junction protein pannexin 3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ96QZ0
  • Protein Name
    Pannexin-3
  • Scientific Description
    Structural component of the gap junctions and the hemichannels.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of PANX3 using anti-PANX3 antibody (A11546-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human A431 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PANX3 antigen affinity purified polyclonal antibody (Catalog # A11546-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PANX3 at approximately 45 kDa. The expected band size for PANX3 is at 45 kDa.
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