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PARD6B Antibody

CSB-PA070032
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetPARD6B
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Overview

  • Supplier
    Cusabio
  • Product Name
    PARD6B Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID84612
  • Target name
    PARD6B
  • Target description
    par-6 family cell polarity regulator beta
  • Target synonyms
    PAR6B, partitioning defective 6 homolog beta, PAR-6 beta, par-6 partitioning defective 6 homolog beta
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9BYG5
  • Protein Name
    Partitioning defective 6 homolog beta
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of PARD6B using anti-PARD6B antibody (A08475-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PARD6B antigen affinity purified polyclonal antibody (Catalog # A08475-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PARD6B at approximately 50 kDa. The expected band size for PARD6B is at 41 kDa.
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