The PARP12 Chemiluminescent Assay Kit is designed to measure PARP12 activity for screening and profiling applications. PARP12 is known to catalyze the NAD-dependent ADP-ribosylation of histones. The key to the PARP12 Chemiluminescent Assay Kit is the biotinylated NAD+. With this kit, only three simple steps are required for PARP12 reactions. First, histone proteins are coated on a 96-well plate. Next, a biotinylated NAD+ mix (termed PARP Substrate Mixture) is incubated with the PARP12 enzyme in an optimized assay buffer. Finally, the plate is treated with streptavidin-HRP followed by addition of the ELISA ECL substrate to produce chemiluminescence that can be measured using a chemiluminescence reader.
