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PARP2 Antibody

HY-P80265
MedChem Express
TargetPARP2
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    PARP2 Antibody
  • Delivery Days Customer
    10
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID10038
  • Target name
    PARP2
  • Target description
    poly(ADP-ribose) polymerase 2
  • Target synonyms
    ADP-ribosyltransferase (NAD+; poly(ADP-ribose) polymerase)-like 2; ADP-ribosyltransferase diphtheria toxin-like 2; ADPRT2; ADPRT-2; ADPRTL2; ADPRTL3; ARTD2; DNA ADP-ribosyltransferase PARP2; hPARP-2; NAD(+) ADP-ribosyltransferase 2; pADPRT-2; PARP-2; poly (ADP-ribose) polymerase family, member 2; poly (ADP-ribosyl) transferase-like 2; poly [ADP-ribose] polymerase 2; poly[ADP-ribose] synthase 2; poly[ADP-ribose] synthetase 2; protein poly-ADP-ribosyltransferase PARP2
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    PARP2 Antibody is a non-conjugated and Rabbit origined monoclonal antibody about 66 kDa, targeting to PARP2. It can be used for WB,FC assays with tag free, in the background of Human.
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PARP2 using anti-PARP2 antibody (A03270-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat testis tissue lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: human HL-60 whole cell lysates, Lane 4: human U20S whole cell lysates, Lane 5: human HepG2 whole cell lysates, Lane 6: human A431 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PARP2 antigen affinity purified polyclonal antibody (Catalog # A03270-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PARP2 at approximately 66KD. The expected band size for PARP2 is at 66KD.
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