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PARP9 Antibody

LS-C750495
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman
TargetPARP9
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    PARP9 Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 80kDa/92kDa/96kDa.
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:200 - 1:2000) The predicted MW is 80kDa/92kDa/96kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID83666
  • Target name
    PARP9
  • Target description
    poly(ADP-ribose) polymerase family member 9
  • Target synonyms
    ARTD9, BAL, BAL1, MGC:7868, protein mono-ADP-ribosyltransferase PARP9, ADP-ribosyltransferase diphtheria toxin-like 9, PARP-9, b aggressive lymphoma protein, poly (ADP-ribose) polymerase 9, poly [ADP-ribose] polymerase 9
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PARP9 using anti-PARP9 antibody (A08508-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat skeletal muscle tissue lysates, Lane 2: mouse skeletal muscle tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PARP9 antigen affinity purified polyclonal antibody (Catalog # A08508-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PARP9 at approximately 96 kDa. The expected band size for PARP9 is at 96 kDa.
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