PathPlus IGF1R / IGF1 Receptor Antibody (aa1264-1367, clone JBW902)

Biorbyt

PathPlus IGF1R / IGF1 Receptor Antibody (aa1264-1367, clone JBW902)

10

Further information: Immunohistochemistry: LS-B2905 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2905 was determined to be 5 ug/ml. Western Blot: 0.1-2 ug/ml. Detects the beta chain of IGF1R in RIPA lysates from C4 cells (mouse 3T3 cells that have been transfected with the human IGF1R) and in RIPA lysates from mouse 3T3/A31 cells. C4 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with LS-B2905. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Immunoprecipitation: 4 ug/ml immunoprecipitates IGF1R from 1 mg of RIPA lysates from C4 cells (mouse 3T3 cells that have been transfected with the human IGF1R) and in RIPA lysates from mouse 3T3/A31 cells.

IHC, IHC-P (5 µg/ml), IP, WB IHC, IHC-P, IP, WB

Research Use Only

Monoclonal

JBW902

1 mg/ml

Unconjugated

Mouse

IgG2a

PathPlus IGF1R / IGF1 Receptor Antibody (aa1264-1367, clone JBW902) LS-B2905

Human, Mouse

-20°C,2°C to 8°C

12352203