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ProductsBack/PathPlus(tm) ACPP / PAP Antibody

PathPlus(tm) ACPP / PAP Antibody

LS-B16311
LifeSpan BioSciences
ApplicationsImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetACP3
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    PathPlus(tm) ACPP / PAP Antibody
  • Delivery Days Customer
    14
  • Application Supplier Note
    10%-formalin fixed adult mouse tissue recommended for immunohistochemistry.
  • Applications
    ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    IHC, IHC-P (1:500) 10%-formalin fixed adult mouse tissue recommended for immunohistochemistry.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    10 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID55
  • Target name
    ACP3
  • Target description
    acid phosphatase 3
  • Target synonyms
    5'-NT, ACP-3, ACPP, TM-PAP, prostatic acid phosphatase, TMPase, acid phosphatase, prostate, ecto-5'-nucleotidase, prostatic acid phosphotase, protein tyrosine phosphatase ACP3, thiamine monophosphatase
  • Host
    Chicken
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PAP/ACP3 using anti-PAP/ACP3 antibody (A02082-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PAP/ACP3 antigen affinity purified polyclonal antibody (Catalog # A02082-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PAP/ACP3 at approximately 45-50 kDa. The expected band size for PAP/ACP3 is at 45 kDa.
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