PathPlus(tm) BMP7 Antibody (aa315-431)

LifeSpan BioSciences

PathPlus(tm) BMP7 Antibody (aa315-431)

23

Human BMP-7

Immunohistochemistry: LS-B7431 in an IHC-plus antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure). A concentration of 2.5 ug/ml is recommended. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of Human BMP-7 (100 ng/ml), a concentration of 0.4-0.6 ug/ml of this antibody is required. ELISA: To detect hBMP-7 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5-2 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human BMP-7 (LS-C104294) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hBMP-7. Western Blot: To detect hBMP-7 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hBMP-7 is 1.5-3 ng/lane, under either reducing or non-reducing conditions.. ELISA (0.5 - 2 µg/ml), IHC-P (2.5 µg/ml), WB (0.1 - 0.2 µg/ml) Immunohistochemistry: LS-B7431 in an IHC-plus antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure). A concentration of 2.5 ug/ml is recommended. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of Human BMP-7 (100 ng/ml), a concentration of 0.4-0.6 ug/ml of this antibody is required. ELISA: To detect hBMP-7 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5-2 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human BMP-7 (LS-C104294) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hBMP-7. Western Blot: To detect hBMP-7 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hBMP-7 is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin

Research Use Only

Polyclonal

Unconjugated

...

Lyophilized

BMP7

bone morphogenetic protein 7

bone morphogenetic protein 7; OP-1; osteogenic protein 1

Rabbit

IgG

Human

-20°C,2°C to 8°C,RT

12352203