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PathPlus(tm) CASP10 / Caspase 10 Antibody

LS-B16568
LifeSpan BioSciences
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse
TargetCASP10
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    PathPlus(tm) CASP10 / Caspase 10 Antibody
  • Delivery Days Customer
    14
  • Application Supplier Note
    The predicted MW is 28kDa/31kDa/51kDa/54kDa/58kDa, while the observed MW by Western blot was 90kDa.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    IHC-P (1:200), WB (1:500 - 1:2000) The predicted MW is 28kDa/31kDa/51kDa/54kDa/58kDa, while the observed MW by Western blot was 90kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID843
  • Target name
    CASP10
  • Target description
    caspase 10
  • Target synonyms
    ALPS2, FLICE-2, FLICE2, MCH4, caspase-10, CASP-10, FADD-like ICE2, FAS-associated death domain protein interleukin-1B-converting enzyme 2, ICE-like apoptotic protease 4, apoptotic protease MCH-4, caspase 10 apoptosis-related cysteine peptidase, caspase 10, apoptosis-related cysteine protease, interleukin-1B-converting enzyme 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

FAQ

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Figure 1. Western blot analysis of Caspase-10/CASP10 using anti-Caspase-10/CASP10 antibody (A02190-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Caspase-10/CASP10 antigen affinity purified polyclonal antibody (Catalog # A02190-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Caspase-10/CASP10 at approximately 59 kDa. The expected band size for Caspase-10/CASP10 is at 59 kDa.
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