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PathPlus(tm) CD14 Antibody

LS-B16126
LifeSpan BioSciences
ApplicationsImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetCD14
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    PathPlus(tm) CD14 Antibody
  • Delivery Days Customer
    14
  • Application Supplier Note
    Use Heat-induced epitope retrieval (HIER) Citrate buffer of pH 6.0 Recommended IHC controls tissues are Tonsil, Spleen, Lymph node, Appendix, Colon. Resulting localization is Membranous, Cytoplasmic
  • Applications
    ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    IHC (1:200 - 1:400), IHC-P (1:25) Use Heat-induced epitope retrieval (HIER) Citrate buffer of pH 6.0 Recommended IHC controls tissues are Tonsil, Spleen, Lymph node, Appendix, Colon. Resulting localization is Membranous, Cytoplasmic
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID929
  • Target name
    CD14
  • Target description
    CD14 molecule
  • Target synonyms
    monocyte differentiation antigen CD14; myeloid cell-specific leucine-rich glycoprotein
  • Host
    Mouse
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. IHC analysis of CD14 using anti-CD14 antibody (A00137-2). CD14 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 microg/ml rabbit anti-CD14 Antibody (A00137-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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