PathPlus(tm) CFTR Antibody (aa25-36)

LifeSpan BioSciences

PathPlus(tm) CFTR Antibody (aa25-36)

23

WB: Detects CFTR in RIPA lysates from CFTR-transfected BHK cells. Reported to detect CFTR from a human T84 colon carcinoma epithelial cell lysate. CFTR-transfected BHK cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with mouse anti-CFTR (0.5 ug/ml). Proteins were visualized using IgG (HRP) goat anti-mouse secondary antibody and a chemiluminescence detection system. Note: DO NOT BOIL LYSATE. Instead incubate at 37°C for 30 minutes.. ICC: Reported to immunostain CFTR in T84 cells. Note: CFTR runs as a diffuse protein on SDS-PAGE. IP: Immunoprecipitates CFTR from a T84 cell lysate.. ICC, IHC-P (10 µg/ml), IP, WB (0.5 - 2 µg/ml) WB: Detects CFTR in RIPA lysates from CFTR-transfected BHK cells. Reported to detect CFTR from a human T84 colon carcinoma epithelial cell lysate. CFTR-transfected BHK cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with mouse anti-CFTR (0.5 ug/ml). Proteins were visualized using IgG (HRP) goat anti-mouse secondary antibody and a chemiluminescence detection system. Note: DO NOT BOIL LYSATE. Instead incubate at 37°C for 30 minutes.. ICC: Reported to immunostain CFTR in T84 cells. Note: CFTR runs as a diffuse protein on SDS-PAGE. IP: Immunoprecipitates CFTR from a T84 cell lysate.

ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin

Research Use Only

Monoclonal

1 mg/ml

Unconjugated

CFTR

CF transmembrane conductance regulator

ABC35, ABCC7, CF, CFTR/MRP, MRP7, TNR-CFTR, dJ760C5.1, cystic fibrosis transmembrane conductance regulator, cAMP-dependent chloride channel, channel conductance-controlling ATPase, cystic fibrosis transmembrane conductance regulating, cystic fibrosis transmembrane conductance regulator (ATP-binding cassette sub-family C, member 7)

Mouse

IgG1

Human

-20°C,2°C to 8°C

41116161