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ProductsBack/PathPlus(tm) FGA / Fibrinogen Alpha Antibody

PathPlus(tm) FGA / Fibrinogen Alpha Antibody

LS-B16693
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetFGA
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    PathPlus(tm) FGA / Fibrinogen Alpha Antibody
  • Delivery Days Customer
    14
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    IHC-P (5 µg/ml), Peptide-ELISA (1:64000), WB (0.01 - 0.03 µg/ml)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID2243
  • Target name
    FGA
  • Target description
    fibrinogen alpha chain
  • Target synonyms
    Fib2; fibrinogen alpha chain; fibrinogen, A alpha polypeptide
  • Host
    Goat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of FGA using anti-FGA antibody (A00816-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat skeletal muscle tissue lysates, Lane 2: HEPG2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FGA antigen affinity purified polyclonal antibody (Catalog # A00816-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FGA at approximately 95KD. The expected band size for FGA is at 95KD.
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