PathPlus(tm) GPR12 Antibody (Extracellular Domain)

LifeSpan BioSciences

PathPlus(tm) GPR12 Antibody (Extracellular Domain)

23

Human GPR12 / GPCR12. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

Immunohistochemistry: The unconjugated form was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for the unconjugated form was determined to be 10 ug/ml.. IHC, IHC-P (10 µg/ml) Immunohistochemistry: The unconjugated form was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for the unconjugated form was determined to be 10 ug/ml.

ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin

Research Use Only

Polyclonal

0.8 mg/ml

Unconjugated

GPR12

G protein-coupled receptor 12

GPCR12; GPCR21; G-protein coupled receptor 12; PPP1R84; protein phosphatase 1, regulatory subunit 84

Rabbit

Bovine, Canine, Equine, Hamster, Human, Monkey, Mouse, Porcine, Rat

-20°C or -80°C,2°C to 8°C

12352203