PathPlus(tm) GPR3 Antibody (N-Terminus)

LifeSpan BioSciences

PathPlus(tm) GPR3 Antibody (N-Terminus)

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Human GPR3. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except CASKIN2 (53%), INPPL1 (47%).

Immunohistochemistry: The unconjugated form was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for the unconjugated form was determined to be 10 ug/ml.. ELISA, IHC, IHC-P (10 µg/ml) Immunohistochemistry: The unconjugated form was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for the unconjugated form was determined to be 10 ug/ml.

ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin

Research Use Only

Polyclonal

1 mg/ml

Unconjugated

GPR3

G protein-coupled receptor 3

ACCA; ACCA orphan receptor; adenylate cyclase constitutive activator; G-protein coupled receptor 3

Rabbit

Human, Monkey

-20°C or -80°C,2°C to 8°C

12352203