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PathPlus(tm) HEXB Antibody

LS-A10969
LifeSpan BioSciences
ApplicationsImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetHEXB
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    PathPlus(tm) HEXB Antibody
  • Delivery Days Customer
    14
  • Applications
    ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    IHC-P (1.25 µg/ml)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID3074
  • Target name
    HEXB
  • Target description
    hexosaminidase subunit beta
  • Target synonyms
    ENC-1AS, HEL-248, HEL-S-111, beta-hexosaminidase subunit beta, HCC-7, N-acetyl-beta-glucosaminidase subunit beta, beta-N-acetylhexosaminidase subunit beta, beta-hexosaminidase beta-subunit, cervical cancer proto-oncogene 7 protein, epididymis luminal protein 248, epididymis secretory protein Li 111, epididymis secretory sperm binding protein, hexosaminidase B (beta polypeptide), hexosaminidase subunit B
  • Host
    Rabbit
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of HEXB using anti-HEXB antibody (PB9211). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U87 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HEXB antigen affinity purified polyclonal antibody (Catalog # PB9211) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HEXB at approximately 63 kDa. The expected band size for HEXB is at 63 kDa.
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