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ProductsBack/PathPlus(tm) HP / Haptoglobin Antibody

PathPlus(tm) HP / Haptoglobin Antibody

LS-B15349
LifeSpan BioSciences
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetHP
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    PathPlus(tm) HP / Haptoglobin Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 38kDa/45kDa, while the observed MW by Western blot was 45kDa.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    IHC, IHC-P (1:200), WB (1:500 - 1:2000) The predicted MW is 38kDa/45kDa, while the observed MW by Western blot was 45kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID3240
  • Target name
    HP
  • Target description
    haptoglobin
  • Target synonyms
    BP, HP2ALPHA2, HPA1S, haptoglobin, binding peptide, haptoglobin alpha(1S)-beta, haptoglobin alpha(2FS)-beta, haptoglobin, alpha polypeptide, haptoglobin, beta polypeptide, zonulin
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of HP using anti-HP antibody (A00062-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HP antigen affinity purified polyclonal antibody (Catalog # A00062-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HP at approximately 45KD. The expected band size for HP is at 45KD.
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