PathPlus(tm) IGF1 Antibody

LifeSpan BioSciences

PathPlus(tm) IGF1 Antibody

23

Human IGF-I

Immunohistochemistry: LS-B7414 in an IHC-plus antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure). A concentration of 5 ug/ml is recommended. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of hIGF-I (5 ng/ml), a concentration of 0.67-1 ug/ml of this antibody is required. ELISA: To detect hIGF-I by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5-2 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human IGF-I (LS-C104363) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hIGF-I. Western Blot: To detect hIGF-I by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIGF-I is 1.5-3 ng/lane, under either reducing or non-reducing conditions.. ELISA (0.5 - 2 µg/ml), IHC-P (5 µg/ml), WB (0.1 - 0.2 µg/ml) Immunohistochemistry: LS-B7414 in an IHC-plus antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure). A concentration of 5 ug/ml is recommended. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of hIGF-I (5 ng/ml), a concentration of 0.67-1 ug/ml of this antibody is required. ELISA: To detect hIGF-I by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5-2 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human IGF-I (LS-C104363) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hIGF-I. Western Blot: To detect hIGF-I by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIGF-I is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin

Research Use Only

Polyclonal

Unconjugated

Lyophilized

IGF1

insulin like growth factor 1

IGF; IGFI; IGF-I; insulin-like growth factor 1 (somatomedin C); insulin-like growth factor I; insulin-like growth factor IB; mechano growth factor; MGF; somatomedin-C

Rabbit

IgG

Human

-20°C,2°C to 8°C,RT

12352203