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ProductsBack/PathPlus(tm) KCNA2 / Kv1.2 Antibody

PathPlus(tm) KCNA2 / Kv1.2 Antibody

LS-B16482
LifeSpan BioSciences
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse
TargetKCNA2
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    PathPlus(tm) KCNA2 / Kv1.2 Antibody
  • Delivery Days Customer
    14
  • Application Supplier Note
    The predicted MW is 40kDa/56kDa, while the observed MW by Western blot was 60kDa.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    IF (1:50 - 1:100), IHC-P (1:200), WB (1:500 - 1:2000) The predicted MW is 40kDa/56kDa, while the observed MW by Western blot was 60kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1.58 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID3737
  • Target name
    KCNA2
  • Target description
    potassium voltage-gated channel subfamily A member 2
  • Target synonyms
    DEE32, EIEE32, HBK5, HK4, HUKIV, KV1.2, MK2, NGK1, RBK2, potassium voltage-gated channel subfamily A member 2, potassium channel, voltage gated shaker related subfamily A, member 2, potassium voltage-gated channel, shaker-related subfamily, member 2, voltage-gated K(+) channel HuKIV, voltage-gated potassium channel HBK5, voltage-gated potassium channel protein Kv1.2, voltage-gated potassium channel subunit Kv1.2
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

FAQ

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Figure 1. Western blot analysis of Kv1.2 using anti-Kv1.2 antibody (PB9649). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Kidney Tissue Lysate at 50ug, Lane 2: Rat Brain Tissue Lysate at 50ug, Lane 3: Mouse Brain Tissue Lysate at 50ug, Lane 4: Mouse Kidney Tissue Lysate at 50ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Kv1.2 antigen affinity purified polyclonal antibody (Catalog # PB9649) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Kv1.2 at approximately 57 kDa. The expected band size for Kv1.2 is at 57 kDa.
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