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ProductsBack/PathPlus(tm) PACE4 / PCSK6 Antibody (Cytoplasmic Domain)

PathPlus(tm) PACE4 / PCSK6 Antibody (Cytoplasmic Domain)

LS-A8408
LifeSpan BioSciences
ApplicationsELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetPCSK6
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    PathPlus(tm) PACE4 / PCSK6 Antibody (Cytoplasmic Domain)
  • Delivery Days Customer
    23
  • Applications
    ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    ELISA, IHC-P (10 µg/ml)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID5046
  • Target name
    PCSK6
  • Target description
    proprotein convertase subtilisin/kexin type 6
  • Target synonyms
    PACE4, SPC4, proprotein convertase subtilisin/kexin type 6, paired basic amino acid cleaving enzyme 4, paired basic amino acid cleaving system 4, subtilisin-like proprotein convertase 4, subtilisin/kexin-like protease PACE4
  • Host
    Rabbit
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of PACE4 using anti-PACE4 antibody (PB9769). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Brain Tissue Lysate at 50ug, Lane 2: 293T Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PACE4 antigen affinity purified polyclonal antibody (Catalog # PB9769) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PACE4 at approximately 80 kDa, 45/80/100 kDa. The expected band size for PACE4 is at 80 kDa, 45/80/100 kDa.
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