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ProductsBack/PathPlus(tm) RAB5A / RAB5 Antibody (aa182-211)

PathPlus(tm) RAB5A / RAB5 Antibody (aa182-211)

LS-B9629
LifeSpan BioSciences
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetRAB5A
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    PathPlus(tm) RAB5A / RAB5 Antibody (aa182-211)
  • Delivery Days Customer
    23
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    Flo (1:10 - 1:50), IF (1:10 - 1:50), IHC, IHC-P (1:50 - 1:100), WB (1:1000)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5868
  • Target name
    RAB5A
  • Target description
    RAB5A, member RAS oncogene family
  • Target synonyms
    RAB5, ras-related protein Rab-5A, RAS-associated protein RAB5A
  • Host
    Rabbit
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of RAB5A/B/C using anti-RAB5A/B/C antibody (A01891). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U-87MG whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human U251 whole cell lysates, Lane 4: human HL-60 whole cell lysates, Lane 5: rat kidney tissue lysates, Lane 6: rat testis tissue lysates, Lane 7: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RAB5A/B/C antigen affinity purified polyclonal antibody (Catalog # A01891) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RAB5A/B/C at approximately 24 kDa. The expected band size for RAB5A/B/C is at 24 kDa.
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