PathPlus(tm) SERPINF1 / PEDF Antibody (aa196-347)
LS-B2471
ApplicationsWestern Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetSERPINF1
Overview
- SupplierLifeSpan BioSciences
- Product NamePathPlus(tm) SERPINF1 / PEDF Antibody (aa196-347)
- Delivery Days Customer23
- Application Supplier NoteImmunohistochemistry: LS-B2471 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2471 was determined to be 5 ug/ml.. ELISA, ICC, IHC, IHC-Fr, IHC-P (5 µg/ml), WB Immunohistochemistry: LS-B2471 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2471 was determined to be 5 ug/ml.
- ApplicationsWestern Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID5176
- Target nameSERPINF1
- Target descriptionserpin family F member 1
- Target synonymsEPC-1, OI12, OI6, PEDF, PIG35, pigment epithelium-derived factor, alpha-2 antiplasmin, cell proliferation-inducing gene 35 protein, serine (or cysteine) proteinase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 1, serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 1, testis tissue sperm-binding protein Li 70n
- HostMouse
- IsotypeIgG2a
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC41116161

![HepG2 whole cell extract and conditioned medium (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PEDF antibody [N1C2] (GTX114097) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX114097/GTX114097_45364_20240419_WB_Fraction_25070323_566.webp)




