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ProductsBack/PathPlus(tm) SMC1A / SMC1 Antibody (aa1-248)

PathPlus(tm) SMC1A / SMC1 Antibody (aa1-248)

LS-B16485
LifeSpan BioSciences
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityBovine, Human, Mouse
TargetSMC1A
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    PathPlus(tm) SMC1A / SMC1 Antibody (aa1-248)
  • Delivery Days Customer
    14
  • Application Supplier Note
    IHC-paraffin: Suggested antigen retrieval using heat mediated 10 mM Citrate buffer (pH 6.0) or Tris-EDTA buffer (pH 8.0).
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    IHC-P (7.5 µg/ml), WB (1:1000 - 1:10000) IHC-paraffin: Suggested antigen retrieval using heat mediated 10 mM Citrate buffer (pH 6.0) or Tris-EDTA buffer (pH 8.0).
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID8243
  • Target name
    SMC1A
  • Target description
    structural maintenance of chromosomes 1A
  • Target synonyms
    CDLS2, DEE85, DXS423E, EIEE85, SB1.8, SMC1, SMC1L1, SMC1alpha, SMCB, structural maintenance of chromosomes protein 1A, SMC protein 1A, SMC-1-alpha, SMC1 (structural maintenance of chromosomes 1, yeast)-like 1, epididymis secretory sperm binding protein, segregation of mitotic chromosomes 1
  • Host
    Rabbit
  • Reactivity
    Bovine, Human, Mouse
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

FAQ

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Figure 1. Western blot analysis of SMC1A using anti-SMC1A antibody (A02148-5). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SMC1A antigen affinity purified polyclonal antibody (Catalog # A02148-5) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SMC1A at approximately 150 kDa. The expected band size for SMC1A is at 143 kDa.
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