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ProductsBack/PathPlus(tm) TAS1R2 / T1R2 Antibody (N-Terminus)

PathPlus(tm) TAS1R2 / T1R2 Antibody (N-Terminus)

LS-A2007
LifeSpan BioSciences
ApplicationsImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetTAS1R2
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    PathPlus(tm) TAS1R2 / T1R2 Antibody (N-Terminus)
  • Delivery Days Customer
    23
  • Applications
    ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    IHC, IHC-P (5 - 10 µg/ml)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID80834
  • Target name
    TAS1R2
  • Target description
    taste 1 receptor member 2
  • Target synonyms
    GPR71, T1R2, TR2, taste receptor type 1 member 2, G-protein coupled receptor 71, sweet taste receptor T1R2, taste receptor, type 1, member 2
  • Host
    Rabbit
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of TAS1R2 using anti-TAS1R2 antibody (A08729-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PANC-1 whole cell lysates, Lane 2: human Caco-2 whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human COLO 320 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TAS1R2 antigen affinity purified polyclonal antibody (Catalog # A08729-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TAS1R2 at approximately 100 kDa. The expected band size for TAS1R2 is at 95 kDa.
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